Entrance Procedure for Microbiology Area

By: Pharma Tips | Views: 5953 | Date: 03-Oct-2011

To lay down a procedure for entrance in microbiology working area.

1.0 OBJECTIVE
To lay down a procedure for entrance in microbiology working area.

2.0 SCOPE
This SOP is applicable to Quality Control Department Formulation Units

3.0 RESPONSIBILITY
Microbiologist.

4.0 ACCOUNTABILITY
Department Head.

5.0 PREPARATION OF DISINFECTANT SOLUTION.

5.1 Add 25 ml Dettol in 975 ml of purified water in S.S. Bowl to produce 2.5% solution.

5.2 Add 25 ml Savlon in 975 ml of purified water in S.S. Bowl to produce 2.5% solution.

5.3 Add 70ml isopropyl alcohol in 30 ml purified water in Bottle to produce 70% I.P.A. solution and filter through 0.45 micron membrane.

5.4 Daily prepare fresh Savlon and Dettol solution for cleaning and prepare 70% alcohol once a week.

6.0 PROCEDURE

6.1 Remove street shoes and Apron in Ist change room and cross the bench by sitting on it.

6.2 Wash the hands with the Savlon or dettol prepared as above.

6.3 Enter into the II nd change room And wear the sterile Garments as follows, First wear the mask and put a head guard. Then wear the sterile gown followed by Booties.

6.4 Enter into IIIrd change room and put the sterile gloves, wash with 70% I.P.A and enter
into the testing area through cross over bench
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