Difference Between RAMAN Spectra & I.R. Spectra

By: Pharma Tips | Views: 14113 | Date: 24-Apr-2011

RAMAN SPECTRA -It is due to the scattering light by the vibrating molecules.-Polarizability of the molecule will decide whether the Raman spectra will be observed or not.I.R. SPECTRA-It is the result of absorption of light by vibrating molecule.-The presence of permanent dipole moment in a molecule may be regarded as a criterion of I.R. spectra.

Difference   Between  RAMAN Spectra & I.R. Spectra

RAMAN SPECTRA:-                                                 
    It is due to the scattering light by the vibrating molecules.
    Polarizability of the molecule will decide whether the Raman spectra will be observed or not.
    It can be recorded only in one exposure.
    Water can be used as a solvent.
    The method is very accurate but not very sensitive.
    Optical systems are made of glass or quartz.
    Sometimes photochemical reactions take place in the regions of Raman lines & thus create difficulties.
    Substance under investigation must be pure & colourless.
    Vibrational frequencies of large molecule can be measured.
    As Raman lines are weaker in intensity concentrated solution must be used to increase the intensity of Raman lines.
    Homonuclear diatonic molecules are often found to be active.


I.R. SPECTRA:-
    It is the result of absorption of light by vibrating molecule.
    The presence of permanent dipole moment in a molecule may be regarded as a criterion of I.R. spectra.
    It required at least two separate runs with different prisms to cover the whole region of I.R.
    Water cannot be used as solvent because it is opaque to I.R.
    The method is accurate & very sensitive.
    Optical system made up of         special crystals such as  CaF2 , NaBr.
    Photochemical reactions do not take place.
    This condition is not rigid.
    Vibrational frequencies of large molecule cannot be measured.

    Here dilute solutions are preferred.
    Homonuclear diatonic molecules are not found to be active.
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