Advantages and Application of UPLC

By: Pharma Tips | Views: 7698 | Date: 14-May-2011

Ultra-performance liquid chromatography (UPLC), involves HPLC with very high pressures and columns having very small particle sizes.

Advantages of UPLC

-High resolution

-High speed

-It is up to 9 times faster, has up to twice the resolution and three times the sensitivity than that of HPLC

-Quick and ease of instrument handling.

-High sensitivity.

-Even neon particles can be separated easily.

Application of UPLC
-From injection of a poly-drug reference standard and whole blood extract, separation and identification of amphetamine, methamphetamine, ephedrine, pseudoephedrine, phentermine, MDA, MDMA, MDEA and ketamine in less than 3 min using the UPLC method

-Uplc is used for studying hydrolysis kinetics of CL-20 and related energetic compounds.

-Simultaneous Determination of Tetracycline’s and Quinolones Antibiotics in Egg can be done by Ultra-Performance Liquid Chromatography–Electro spray Tandem Mass Spectrometry

-Determination of Coumarone in Food can also be done using Ultra-Performance Liquid Chromatography-Electro spray-Tandem Mass Spectrometry .

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